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Laboratory of Food Quality Design and Developmen , Division of Agronomy and Horticultural Science , Graduate School of Agriculture, Kyoto University


  • Expression and characterization of protein disulfide isomerase family proteins in bread wheat.
    Kimura S, Higashino Y, Kitao Y, Masuda T, Urade R
    BMC Plant Biol 15, 460 (2015).

  • Preliminary X-ray analysis of the binding domain of the soybean vacuolar sorting receptor complexed with a sorting determinant of a seed storage protein.
    Maruyama N, Goshi T, Sugiyama S, Niiyama M, Adachi H, Takano K, Murakami S, Inoue T, Mori Y, Matsumura H, Mikami B
    Acta Crystallogr F Struct Biol Commun 71, 132-135 (2015).

  • The iron content and ferritin contribution in fresh, dried, and toasted nori, Pyropia yezoensis.
    Masuda T, Yamamoto A, Toyohara H
    Biosci Biotechnol Biochem 79, 74-81 (2015).

  • Crystal structure of class III chitinase from pomegranate provides the insight into its metal storage capacity.
    Masuda T, Zhao G, Mikami B
    Biosci Biotechnol Biochem 79, 45-50 (2015).

  • Disulfide bond formation activity of soybean quiescin sulfhydryl oxidase.
    Okuda A, Matsusaki M, Higashino Y, Masuda T, Urade R
    FEBS J 281, 5341-5355 (2014).

  • Knockdown of the 7S globulin subunits shifts distribution of nitrogen sources to the residual protein fraction in transgenic soybean seeds.
    Yamada T, Mori Y, Yasue K, Maruyama N, Kitamura K, Abe J
    Plant Cell Rep 33,1963-1976 (2014).

  • Stable accumulation of seed storage proteins containing vaccine peptides in transgenic soybean seeds.
    Maruyama N, Fujiwara K, Yokoyama K, Cabanos C, Hasegawa H, Takagi K, Nishizawa K, Uki Y, Kawarabayashi T, Shouji M, Ishimoto M, Terakawa T
    J Biosci Bioeng 118, 441-447 (2014).

  • Development of a novel transgenic rice with hypocholesterolemic activity via high-level accumulation of the α' subunit of soybean β-conglycinin.
    Cabanos C, Kato N, Amari Y, Fujiwara K, Ohno T, Shimizu K, Goto T, Shimada M, Kuroda M, Masuda T, Takaiwa F, Utsumi S, Nagaoka S, Maruyama N
    Transgenic Res 23, 609-620 (2014).

  • Differential expression and elution behavior of basic 7S globulin among cultivars under hot water treatment of soybean seeds.
    Fujiwara K, Cabanos C, Toyota K, Kobayashi Y, Maruyama N
    J Biosci Bioeng 117, 742-748 (2014).

  • The crystal structure of a crustacean prophenoloxidase provides a clue to understanding the functionality of the type 3 copper proteins.
    Masuda T, Momoji K, Hirata T, Mikami B
    FEBS J 281, 2659-2673 (2014).

  • Role of cysteine-protease CGHC motifs of ER-60, a protein disulfide isomerase, in hepatic apolipoprotein B100 degradation.
    Rutledge AC, Qiu W, Zhang R, Urade R, Adeli K
    Arch Biochem Biophys 537, 104-112 (2013).

  • Degradation of β-conglycinin β-homotrimer by papain: independent occurrence of limited and extensive proteolysis.
    Shutov AD, Rudakova AS, Rudakov SV, Kakhovskaya IA, Schallau AA, Wilson KA, Maruyama N
    Biosci Biotechnol Biochem 77, 2082-2086 (2013).

  • Purification, crystallization and preliminary crystallographic analysis of soybean mature glycinin A1bB2.
    Prak K, Mikami B, Itoh T, Fukuda T, Maruyama N, Utsumi S
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69, :937-941 (2013).

  • Modification of solubility and heat-induced gelation of amaranth 11S globulin by protein engineering.
    Carrazco-Peña L, Osuna-Castro JA, De León-Rodríguez A, Maruyama N, Toro-Vazquez JF, Morales-Rueda JA, Barba de la Rosa AP
    J Agric Food Chem 61, 3509-3516 (2013).

  • Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.
    Morimoto S, Tomohiro T, Maruyama N, Hatanaka Y
    Chem Commun (Camb) 49, 1811-1813 (2013).

  • High-level production of lactostatin, a hypocholesterolemic peptide, in transgenic rice using soybean A1aB1b as carrier.
    Cabanos C, Ekyo A, Amari Y, Kato N, Kuroda M, Nagaoka S, Takaiwa F, Utsumi S, Maruyama N.
    Transgenic Res 22, 621-629 (2013).

  • Mass spectrometry based N- and C-terminal sequence determination of a hepatopancreas-type prophenoloxidase from the kuruma prawn, Marsupenaeus japonicus.
    Kuyama H, Masuda T, Nakajima C, Momoji K, Sugawara T, Nishimura O, Hirata T
    Anal Bioanal Chem 405, 2333-2340 (2013).

  • Accumulation of β-conglycinin in soybean cotyledon through the formation of disulfide bonds between α'- and α-subunits.
    Wadahama H, Iwasaki K, Matsusaki M, Nishizawa K, Ishimoto M, Arisaka F, Takagi K, Urade R
    Plant Physiol 158, 1395-1405 (2012).

  • Crystal structure of a major seed storage protein, 11S proglobulin, from Amaranthus hypochondriacus: insight into its physico-chemical properties.
    Tandang-Silvas MR, Cabanos CS, Carrazco Peña LD, De la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B, Maruyama N
    Food Chem 135, 819-826 (2012).

  • Measurement of Ara h 1-, 2-, and 3-specific IgE antibodies is useful in diagnosis of peanut allergy in Japanese children.
    Ebisawa M, Movérare R, Sato S, Maruyama N, Borres MP, Komata T
    Pediatr Allergy Immunol 23, 573-581 (2012).

  • Limited proteolysis regulates massive degradation of glycinin, storage 11S globulin from soybean seeds: an in vitro model.
    Shutov A, Rudakova A, Rudakov S, Kakhovskaya I, Schallau A, Maruyama N, Wilson K
    J Plant Physiol 169, 1227-1233 (2012).

  • Identification and characterization of a ferritin gene and its product from the multicellular green alga Ulva pertusa.
    Morimoto S, Masuda T, Sugihara I, Toyohara H
    Biosci Biotechnol Biochem 76, 1913-1919 (2012).

  • A novel strategy of natural plant ferritin to protect DNA from oxidative damage during iron oxidation.
    Liao X, Lv C, Zhang X, Masuda T, Li M, Zhao G
    Free Radic Biol Med 53, 375-382 (2012).

  •  The extension peptide of plant ferritin from sea lettuce contributes to shell stability and surface hydrophobicity.
    Masuda T, Morimoto S, Mikami B, Toyohara H
    Protein Sci 21, 786-796 (2012).
  • A novel type of prophenoloxidase from the kuruma prawn Marsupenaeus japonicus contributes to the melanization of plasma in crustaceans.
    Masuda T, Otomo R, Kuyama H, Momoji K, Tonomoto M, Sakai S, Nishimura O, Sugawara T, Hirata T
    Fish Shellfish Immunol 32, 61-68 (2012).

  • Molecular design of seed storage proteins for enhanced food physicochemical properties.
    Tandang-Silvas MR, Tecson-Mendoza EM, Mikami B, Utsumi S, Maruyama N.
    Annu Rev Food Sci Technol 2, 59-73 (2012).

  • Crystal structure of the major peanut allergen Ara h 1.
    Cabanos C, Urabe H, Tandang-Silvas MR, Utsumi S, Mikami B, Maruyama N
    Mol Immunol 49, 115-123 (2011).

  • Expression and purification of peanut oleosins in insect cells.
    Cabanos C, Katayama H, Tanaka A, Utsumi S, Maruyama N
    Protein J 30, 457-463 (2011).

  • The development of transgenic crops to improve human health by advanced utilization of seed storage proteins.
    Maruyama N, Mikami B, Utsumi S
    Biosci Biotechnol Biochem 75, 823-828 (2011). Review.

  • Chitinase III in pomegranate seeds (Punica granatum Linn.): a high-capacity calcium-binding protein in amyloplasts.
    Yang H, Zhang T, Masuda T, Lv C, Sun L, Qu G, Zhao G
    Plant J 68, 765-776 (2011).

  • High-capacity calcium-binding chitinase III from pomegranate seeds (Punica granatum Linn.) is located in amyloplasts.
    Lv C, Masuda T, Yang H, Sun L, Zhao G.

    Plant Signal Behav 6, 1963-1965 (2011). Review.
  • Relationship between endogenous protein disulfide isomerase family proteins and glutenin macropolymer.
    Koh A, Nishimura K & Urade R
    J Agric Food Chem 58,12970-12975 (2010).
  • Crystallization and preliminary X-ray analysis of the major peanut allergen Ara h 1 core region.
    Cabanos C, Urabe H, Masuda T, Tandang-Silvas MR, Utsumi S, Mikami B & Maruyama N
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66, 1071-1073 (2010).

    The universal mechanism for iron translocation to the ferroxidase site in ferritin, which is mediated by the well conserved transit site.
    Masuda T, Goto F, Yoshihara T & Mikami B
    Biochem Biophys Res Commun 400, 94-99 (2010).
  • Role of H-1 and H-2 subunits of soybean seed ferritin in oxidative deposition of iron in protein.
    Deng J, Liao X, Yang H, Zhang X, Hua Z, Masuda T, Goto F, Yoshihara T & Zhao G
    J Biol Chem 285, 32075-32086 (2010).
  • Soybean basic 7S globulin: subunit heterogeneity and molecular evolution.
    Shutov AD, Prak K, Fukuda T, Rudakov SV, Rudakova AS, Tandang-Silvas MR, Fujiwara K, Mikami B, Utsumi S & Maruyama N
    Biosci Biotechnol Biochem 74,1631-1634 (2010).
  • Expression, purification and preliminary crystallization of amaranth 11S proglobulin seed storage protein from Amaranthus hypochondriacus L.
    Tandang-Silvas MR, Carrazco-Pena L, Barba de la Rosa AP, Osuna-Castro JA, Utsumi S, Mikami B & Maruyama N
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66, 919-922 (2010).
  • Development of transgenic rice containing a mutated beta subunit of soybean beta-conglycinin for enhanced phagocytosis-stimulating activity.
    Motoyama T, Amari Y, Tandang-Silvas MR, Cabanos C, Kimura A, Yoshikawa M, Takaiwa F, Utsumi S & Maruyama N
    Peptides 31, 1245-1250 (2010).
  • Expression, purification, cross-reactivity and homology modeling of peanut profilin.
    Cabanos C, Tandang-Silvas MR, Odijk V, Brostedt P, Tanaka A, Utsumi S & Maruyama N
    Protein Expr Purif 73, 36-45 (2010).
  • Conservation and divergence on plant seed 11S globulins based on crystal structures.
    Tandang-Silvas MR, Fukuda T, Fukuda C, Prak K, Cabanos C, Kimura A, Itoh T, Mikami B, Utsumi S & Maruyama N
    Biochim Biophys Acta 1804, 1432-1442 (2010).
  • Carbohydrate moieties contribute significantly to the physicochemical properties of french bean 7s globulin phaseolin.
    Kimura A, Tandang-Silvas MR, Fukuda T, Cabanos C, Takegawa Y, Amano M, Nishimura S, Matsumura Y, Utsumi S & Maruyama N
    J Agric Food Chem 58, 2923-2930 (2010).
  • Co-expression of alpha' and beta subunits of beta-conglycinin in rice seeds and its effect on the accumulation behavior of the expressed proteins.
    Motoyama T, Okumoto Y, Tanisaka T, Utsumi S & Maruyama N
    Transgenic Res 19, 819-827 (2010).
  • A novel EP-involved pathway for iron release from soya bean seed ferritin.
    Fu X, Deng J, Yang H, Masuda T, Goto F, Yoshihara T & Zhao G
    Biochem J 427, 313-321 (2010).
  • Crystal structure of plant ferritin reveals a novel metal binding site that functions as a transit site for metal transfer in ferritin.
    Masuda T, Goto F, Yoshihara T & Mikami B
    J Biol Chem 285, 4049-4059 (2010).
  • Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs.
    Iwasaki K, Kamauchi S, Wadahama H, Ishimoto M, Kawada T & Urade R
    FEBS J 276, 4130-4141 (2009).
  • The endoplasmic reticulum stress signaling pathways in plants.
    Urade R
    Biofactors, 35, 326-331 (2009).
  • Changes in lipid metabolism by soy b-conglycinin-derived peptides in HepG2 cells.
    Mochizuki Y, Maebuchi M, Kohno M, Hirotsuka M, Wadahama H, Moriyama T, Kawada T & Urade R
    J Agric Food Chem 57,1473-1480 (2009).
  • Molecular cloning and characterization of two soybean protein disulfide isomerases as molecular chaperones for seed storage proteins.
    Kamauchi S, Wadahama H, Iwasaki K, Nakamoto Y, Nishizawa K, Ishimoto M, Kawada T & Urade R
    FEBS J 275, 2644-2658 (2008).
  • A novel plant protein disulfide isomerase family homologous to animal P5 - molecular cloning and characterization as a functional protein for folding of soybean seed-storage proteins.
    Wadahama H, Kamauchi S, Nakamoto Y, Nishizawa K, Ishimoto M, Kawada T & Urade R
    FEBS J 275, 399-410 (2008).
  • Disaggregation and reaggregation of gluten proteins by sodium chloride.
    Ukai T, Matsumura Y & Urade R
    J Agric Food Chem 56,1122-1130 (2008).
  • Aggregation of proteins having Golgi apparatus determinant induces large globular structures derived from the endoplasmic reticulum in plant seed cells.
    Maruyama N, Okuda E, Tatsuhara M & Utsumi S
    FEBS Lett 582,1599-1606 (2008).
  • Activation of caspase 3 in HepG2 cells by elaidic acid (t18:1).
    Kondoh Y, Kawada T & Urade R
    Biochim Biophys Acta 1771, 500-505 (2007).
  • Cellular response to unfolded proteins in the endoplasmic reticulum of plants.
    Urade R
    FEBS J 274,1152-1171 (2007).
  • Protein disulfide isomerase family proteins involved in soybean protein biogenesis. Wadahama H, Kamauchi S, Ishimoto M, Kawada T & Urade R
    FEBS J 274, 687-703 (2007).
  • Construction of homo- and heteropolymers of plant ferritin subunits using an in vitro protein expression system.
    Masuda T, Goto F, Yoshihara T, Ezure T, Suzuki T, Kobayashi S, Shikata M & Utsumi S
    Protein Expr Purify 56, 237-246 (2007).
  • The C-terminal region of ƒ¿Œ subunit of soybean ƒÀ-conglycinin contains two types of vacuolar sorting determinants.
    Nishizawa K, Maruyama N & Utsumi S
    Plant Mol Biol 62, 111-125 (2006).
  • Multiple vacuolar sorting determinants sxist in soybean 11S globulin.
    Maruyama,N., Loeng CM, Tatsuhara M, Sawada M, Ishimoto M & Utsumi S
    Plant Cell 18, 1253-1273 (2006).
  • Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana.
    Kamauchi S, Nakatani H, Nakano C & Urade R
    FEBS J 272, 3461-3476 (2005).